Journal
/
生物工程
/
Detection of Endotoxin in Nano-formulations Using Limulus Amoebocyte Lysate (LAL) Assays
JoVE 杂志
生物工程
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 生物工程
Detection of Endotoxin in Nano-formulations Using Limulus Amoebocyte Lysate (LAL) Assays

Detection of Endotoxin in Nano-formulations Using Limulus Amoebocyte Lysate (LAL) Assays

DOI:
10.3791/58830-v

•

06:15 min

•

January 30, 2019

•

,
1Nanotechnology Characterization Lab., Cancer Research Technology Program,Frederick National Laboratory for Cancer Research sponsored by National Cancer Institute

Chapters

  • 00:04Title
  • 00:32Turbidity Limulus Amoebocyte Lysate (LAL) Assay
  • 02:26Sample Loading and Preparation and Gel-Clot LAL
  • 04:27Results: Representative Detection of Endotoxin in PEGylated Liposomal Doxorubicin Using LAL Assays
  • 05:12Conclusion

Summary

自动翻译

自动翻译

Detection of endotoxins in engineered nanomaterials represents one of the grand challenges in the field of nanomedicine. Here, we present a case study that describes the framework composed of three different LAL formats to estimate potential endotoxin contamination in nanoparticles.

Tags

Keywords: EndotoxinNano-formulationsLimulus Amoebocyte Lysate (LAL) AssaysCalibration StandardControl Standard EndotoxinLAL Grade WaterQuality ControlInstrument SettingsTest IDData GroupHardwareLAL MethodSerial NumberSystem IDSerial PortSample IDNegative ControlStandard CurveTest SamplesInhibition Enhancement ControlDilution

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

check_url/cn/58830?article_type=v

Developing Custom Chinese Hamster Ovary-host Cell Protein Assays using Acoustic Membrane Microparticle Technology
check_url/cn/58830?article_type=v

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules
check_url/cn/58830?article_type=v

Detection and Isolation of Circulating Melanoma Cells using Photoacoustic Flowmetry
check_url/cn/58830?article_type=v

Bacterial Detection & Identification Using Electrochemical Sensors
check_url/cn/58830?article_type=v

Flexural Rigidity Measurements of Biopolymers Using Gliding Assays
check_url/cn/58830?article_type=v

Quantification of Global Diastolic Function by Kinematic Modeling-based Analysis of Transmitral Flow via the Parametrized Diastolic Filling Formalism
check_url/cn/58830?article_type=v

Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules
check_url/cn/58830?article_type=v

Eye Irritation Test (EIT) for Hazard Identification of Eye Irritating Chemicals using Reconstructed Human Cornea-like Epithelial (RhCE) Tissue Model
check_url/cn/58830?article_type=v

Label-free Single Molecule Detection Using Microtoroid Optical Resonators
check_url/cn/58830?article_type=v

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Read Article