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Developmental Biology

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JoVE Journal Developmental Biology
I Vitro Generation af Somite derivater fra menneskelige induceret celler pluripotente stamceller
 
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I Vitro Generation af Somite derivater fra menneskelige induceret celler pluripotente stamceller

Article DOI: 10.3791/59359-v • 06:21 min • April 25th, 2019
April 25th, 2019 •
1, 2, 2
1Department of Life Science Frontiers, Center for iPS Cells Research and Application, Kyoto University, 2Department of Clinical Application, Center for iPS Cells Research and Application, Kyoto University

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Vi præsenterer her en protokol for differentiering af menneskelige inducerede pluripotente stamceller i hver somite derivat (myotome, sclerotome, dermatome og syndetome) i kemisk definerede betingelser, som har applikationer i fremtidige sygdom modellering og celle-baserede behandlinger i ortopædisk kirurgi.

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Udviklingsmæssige biologi spørgsmålet 146 induceret pluripotente stamceller differentiering paraksiale mesoderm somite myotome sclerotome dermatome syndetome
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