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生物化学
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A Colorimetric Assay of Citrate Synthase Activity in Drosophila Melanogaster
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生物化学
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JoVE 杂志 生物化学
A Colorimetric Assay of Citrate Synthase Activity in Drosophila Melanogaster

A Colorimetric Assay of Citrate Synthase Activity in Drosophila Melanogaster

DOI:
10.3791/59454-v

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04:30 min

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January 16, 2020

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, , ,
1Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai Clinical Center for Diabetes,Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, 2Department of Anatomy of Physiology,Shanghai Jiao Tong University School of Medicine

Chapters

  • 00:04Title
  • 00:38Colorimetric Citrate Synthase Activity Assay
  • 02:46Results: Effect of dRNF34 and dPGC-1 Knockdown on Citrate Synthase Activity
  • 03:38Conclusion

Summary

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We present a protocol for a colorimetric assay of citrate synthase activity for quantification of intact mitochondrial mass in Drosophila tissue homogenates.

Tags

Citrate SynthaseColorimetric AssayDrosophila MelanogasterMitochondrial FunctionExtraction BufferProtein ConcentrationEnzymatic ActivityDRNF34DPGC-1

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