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Rapid Generation of Primary Murine Melanocyte and Fibroblast Cultures
JoVE 杂志
癌症研究
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JoVE 杂志 癌症研究
Rapid Generation of Primary Murine Melanocyte and Fibroblast Cultures
DOI:

06:32 min

June 26, 2019

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Chapters

  • 00:04Title
  • 00:59Melanocyte and Fibroblast Isolation
  • 04:46Results: Analysis of the Generated Primary Murine Melanocyte and Fibroblast Cultures
  • 06:13Conclusion

Summary

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This protocol outlines a rapid method to simultaneously generate melanocyte and fibroblast cultures from the skin of 0-4 day old mice. These primary cultures can be maintained and manipulated in vitro to study a variety of physiologically relevant processes, including skin cell biology, pigmentation, wound healing and melanoma.

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