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Immunology and Infection

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JoVE Journal Immunology and Infection
Lentiviral CRISPR/Cas9-Mediated Genome Editing for the Study of Hematopoietic Cells in Disease Models
 

Lentiviral CRISPR/Cas9-Mediated Genome Editing for the Study of Hematopoietic Cells in Disease Models

Article DOI: 10.3791/59977-v • 08:14 min • October 3rd, 2019
October 3rd, 2019 •
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1Hematovascular Biology Center, Robert M. Berne Cardiovascular Research Center, University of Virginia School of Medicine

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Described are protocols for the highly efficient genome editing of murine hematopoietic stem and progenitor cells (HSPC) by the CRISPR/Cas9 system to rapidly develop mouse model systems with hematopoietic system-specific gene modifications.

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Lentiviral CRISPR/Cas9 Genome Editing Hematopoietic Cells Disease Models Gene Manipulation Lentivirus-mediated Transgene Delivery Systems Animal Models Mutations Transgenic Approaches Research Procedure Ying Wang 293T Cells Transfection Plasmids Lentivirus Vector PsPAX2 PMD2.G PEI MAX DMEM
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