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JoVE Journal Bioengineering
Crecimiento de capas celulares endoteliales corneales humanas y ovinas en membranas biomateriales
 
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Crecimiento de capas celulares endoteliales corneales humanas y ovinas en membranas biomateriales

Article DOI: 10.3791/60762-v • 05:20 min • February 6th, 2020
February 6th, 2020 •
1, 2, 1, 1,3,4,5,6,7,8, 1,3,4
1Queensland Eye Institute, 2King Faisal University, 3School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, 4Institute of Health and Biomedical Innovation, Queensland University of Technology, 5Science and Engineering Faculty, Queensland University of Technology, 6Faculty of Medicine, University of Queensland, 7Australian Institute for Bioengineering and Nanotechnology, University of Queensland, 8Faculty of Science, University of Western Australia

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Este protocolo describe los pasos críticos necesarios para establecer y cultivar cultivos celulares endoteliales corneales a partir de explantas de tejido humano o ovino. También se presenta un método para subculturizar células endoteliales corneales en biomateriales membranosas.

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Bioingeniería Número 156 endotelio corneal córnea membrana de Descemet explanta membrana biomaterial cámara de cultivo
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