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Neuroscience

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JoVE Journal Neuroscience
Preparation of Acute Human Hippocampal Slices for Electrophysiological Recordings
 

Preparation of Acute Human Hippocampal Slices for Electrophysiological Recordings

Article DOI: 10.3791/61085-v • 07:31 min • May 7th, 2020
May 7th, 2020 •
1, 1,2, 3, 3, 3, 1, 1,2
1Department of Neurology with Experimental Neurology, Charité-Universitätsmedizin Berlin, Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 2NeuroCure Cluster of Excellence, Charité-Universitätsmedizin Berlin, Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 3Department of Neurosurgery, Charité-Universitätsmedizin Berlin, Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health

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Automatic Translation

The presented protocol describes the transport and preparation of resected human hippocampal tissue with the ultimate goal to use vital brain slices as a preclinical evaluation tool for potential antiepileptic substances.

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Acute Human Hippocampal Slices Electrophysiological Recordings Anti-epileptic Substances Drug Development Temporal Lobe Epilepsy Choline ACSF Solution Glucose Calcium Chloride Osmolarity Carbogen Gas Glass Gas Dispenser Storage ACSF High Potassium Plus 4-aminopyridine ACSF Low Magnesium Plus Bicuculline Interface Chamber Filter Paper Slice-holding Compartment
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