/

化学

/

直接耦合电雷电图（DC-ERG），用于记录小鼠视网膜颜料皮皮的光唤起电响应

JoVE 杂志
化学

需要订阅 JoVE 才能查看此. 登录或开始免费试用。

JoVE 杂志化学

Direct-Coupled Electroretinogram (DC-ERG) for Recording the Light-Evoked Electrical Responses of the Mouse Retinal Pigment Epithelium

Please note that all translations are automatically generated. Click here for the English version.

直接耦合电雷电图（DC-ERG），用于记录小鼠视网膜颜料皮皮的光唤起电响应

DOI:

10.3791/61491-v

•

09:10 min

•

July 14, 2020

•

Kiyoharu J. Miyagishima, Congxiao Zhang*², Volha V. Malechka*³, Kapil Bharti, Wei Li

¹Retinal Neurophysiology Section, National Eye Institute,National Institutes of Health, ²Ocular and Stem Cell Translational Research Section, National Eye Institute,National Institutes of Health, ³Human Visual Function Core, National Eye Institute,National Institutes of Health

Chapters

00:04Introduction
00:44Capillary Electrode Filling
01:51Microelectrode Holder Preparation
02:46Electrode Test
04:08Mouse and Electrode Positioning
06:15Direct-Coupled Electroretinogram (DC-ERG) Recording
07:04Results: Representative DC-ERG Traces and Workflow Analyses
08:25Conclusion

Summary

自动翻译

在这里，我们提出了一种方法，使用马莫斯坦、佩奇和同事在21世纪早期首次描述的一种称为DC-ERG的技术，记录小鼠视网膜色素上皮（RPE）的光唤起电反应。

Tags

Keywords: Direct-coupled Electroretinogram DC-ERG Retinal Pigment Epithelium Non-invasive Evaluation Age-related Changes Disease Progression Pharmacological Intervention Reproducibility Capillary Electrode HBSS Vacuum Chamber Custom Stand Microelectrode Holder T-clip Magnetic Ball Joint Electrode Test Ground Electrode Reference Electrode

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

LabVIEW操作的新型纳升渗透压仪冰结合蛋白的调查

生产二硫键稳定的跨膜肽复合物的结构研究

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D ¹H NMR

A High-throughput-compatible FRET-based Platform for Identification and Characterization of Botulinum Neurotoxin Light Chain Modulators

生物分子有序结构由短肽的自组装形成

优化合成蛋白质：Interpositional依赖性的鉴定指示在结构和/或功能性连接的残留

蛋白质生物缀合物的合成<em>通过</em>半胱氨酸马来酰亚胺化学

使用Photoredox催化剂蠕虫状胶束的简便合成可见光介导的分散聚合

利用毛细管电泳从植物组织量化有机酸：一个测试用例检查<em>小粒种咖啡</em>种子

洞察氨基酸和多肽与无机材料的使用相互作用的单分子力谱

Read Article