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JoVE Journal Developmental Biology
Generation of 3D Whole Lung Organoids from Induced Pluripotent Stem Cells for Modeling Lung Developmental Biology and Disease
 

Generation of 3D Whole Lung Organoids from Induced Pluripotent Stem Cells for Modeling Lung Developmental Biology and Disease

Article DOI: 10.3791/62456-v • 09:45 min • April 12th, 2021
April 12th, 2021 •
1,2,3, 2,3, 2,3, 1,2,3
1Department of Pediatrics, University of California, San Diego School of Medicine, 2Sanford Consortium for Regenerative Medicine, 3Sanford Burnham Prebys Medical Discovery Institute

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The article describes step wise directed differentiation of induced pluripotent stem cells to three-dimensional whole lung organoids containing both proximal and distal epithelial lung cells along with mesenchyme.

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3D Whole Lung Organoids Induced Pluripotent Stem Cells Lung Developmental Biology Lung Disease Modeling Human Lung Tissue Differentiation Cell Culture Cryopreservation Genetic Manipulation Growth Factor Reduced Basement Membrane Matrix Medium DMEM F12 P1000 Tips 12 Well Plate GFR Basement Membrane Matrix Medium ROCK Inhibitor Y27632
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