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Longitudinal Follow-Up of Urinary Tract Infections and Their Treatment in Mice using Bioluminescence Imaging
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JoVE 杂志 免疫与感染
Longitudinal Follow-Up of Urinary Tract Infections and Their Treatment in Mice using Bioluminescence Imaging

Longitudinal Follow-Up of Urinary Tract Infections and Their Treatment in Mice using Bioluminescence Imaging

DOI:
10.3791/62614-v

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07:39 min

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June 14, 2021

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, , , , , , ,
1Laboratory of Ion Channel Research (LICR), VIB-KU Leuven Center for Brain & Disease Research, Leuven, Belgium & Department of Cellular and Molecular Medicine,KU Leuven, 2Biomedical MRI, Department of Imaging & Pathology,KU Leuven, 3Belgium & KU Leuven Centre of Microbial and Plant Genetics,VIB-KU Leuven Center for Microbiology, Leuven, 4Laboratory of Experimental Urology, Department of Development and Regeneration,KU Leuven

Chapters

  • 00:04Introduction
  • 00:57Culturing Bacteria
  • 02:36Inoculation of the Animals
  • 04:04Bioluminescence Imaging
  • 05:49Results: The Analysis of BLI Correlation with CFU at the Time of Instillation and Longitudinal Follow-up During Treatment
  • 07:02Conclusion

Summary

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This manuscript describes the intravesical administration of uropathogenic bacteria with a lux operon to induce a urinary tract infection in mice and subsequent longitudinal in vivo analysis of the bacterial load using bioluminescence imaging.

Tags

Keywords: Bioluminescence ImagingUrinary Tract InfectionsUTILongitudinal Follow-upBacterial CultureInoculum PreparationAnesthesiaIntravesical Instillation

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