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JoVE Journal Cancer Research
Defining Gene Functions in Tumorigenesis by Ex vivo Ablation of Floxed Alleles in Malignant Peripheral Nerve Sheath Tumor Cells
 

Defining Gene Functions in Tumorigenesis by Ex vivo Ablation of Floxed Alleles in Malignant Peripheral Nerve Sheath Tumor Cells

Article DOI: 10.3791/62740-v • 09:37 min • August 25th, 2021
August 25th, 2021 •
1, 2,3, 1
1Department of Pathology and Laboratory Medicine, Medical University of South Carolina, 2Department of Pediatrics and Neurology, Perelman School of Medicine of the University of Pennsylvania, 3Division of Child Neurology, Children's Hospital of Philadelphia

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Here, we present a protocol for performing gene knockouts that are embryonic lethal in vivo in genetically engineered mouse model-derived tumors and then assessing the effect that the knockout has on tumor growth, proliferation, survival, migration, invasion, and the transcriptome in vitro and in vivo.

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Gene Functions Tumorigenesis Ex Vivo Ablation Floxed Alleles Malignant Peripheral Nerve Sheath Tumor Cells Embryonic Lethal Gene Knockout In Vivo Method Tumor Growth Role Genetically Engineered Mouse Model Loss Of Function Effects Diseases Embryonic Essential Genes Traditional Gene Knockout Mouse Models Animal Model Generation Adenoviral Infection Recombination Tumor Dissection Formalin Fixation Paraffin Embedding Early Passage Culture Generation Flash Freezing Analytical Analyses Hematoxylin And Eosin Staining Immunohistochemical Staining
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