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Development of pHluorin2-Based Sensor for Glycosomal pH in Trypanosoma brucei

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测量布氏活锥虫的动态糖体 pH 值变化

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Measuring Dynamic Glycosomal pH Changes in Living Trypanosoma brucei

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测量布氏活锥虫的动态糖体 pH 值变化

Development of pHluorin2-Based Sensor for Glycosomal pH in Trypanosoma brucei

DOI:

10.3791/66279-v

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01:46 min

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January 19, 2024

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Daniel Call*¹, Sabrina S. Pizarro*^2,3, Erica Tovey, Emily Knight³, Carrie Baumgardner⁴, Kenneth A. Christensen, James C. Morris

¹Department Chemistry and Biochemistry,Brigham Young University, ²Eukaryotic Pathogens Innovation Center,Clemson University, ³Department of Genetics and Biochemistry,Clemson University, ⁴Department of Physics and Astronomy,Clemson University

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Keywords: Trypanosoma Brucei Glycosome PH Biosensor PHluorin2 Glucose Metabolism Flow Cytometry PH Regulation Glycolysis Hexokinase Phosphofructokinase

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