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Biologie
Construct Design for Transfecting Cysteine-Engineered Voltage-Gated Ca2+ Channels in Murine Isolated Skeletal Muscle Fibers (Video) | JoVE
Published: June 02, 2023
doi:
10.3791/200065
Hugo Bibollet
,
Daniel F. Bennett
,
Martin F. Schneider
,
Erick O. Hernández-Ochoa
1
Department of Biochemistry and Molecular Biology
,
University of Maryland School of Medicine
Tags
Keywords: Cysteine-engineered Voltage-gated Ca2+ Channels
Murine Isolated Skeletal Muscle Fibers
CaV1.1 CDNA Construct
EGFP-tagged Alpha Subunit
Cytomegalovirus Promoter
Site-directed Mutagenesis
S4 Membrane Extracellular Interface
GFP Observation
Ionic Current Recording
check_url/de/200065?article_type=t
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Construct Design for Transfecting Cysteine-Engineered Voltage-Gated Ca
2+
Channels in Murine Isolated Skeletal Muscle Fibers.
J. Vis. Exp.
(Pending Publication), e200065, doi:10.3791/200065 (2023).
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