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Biologie
Murine Adipose-Derived Mesenchymal Cell Isolation, Maintenance and Characterization (Video) | JoVE
Published: April 07, 2023
doi:
10.3791/200083
Aymé Oliva Cárdenas
2
,
Blanca C. Zamora-Rodríguez
,
Kevin A. Batalla-García
,
Alejandro Ávalos-Rodríguez
,
Alejandra Contreras-Ramos
,
Clara Ortega-Camarillo
1
Doctorado en Ciencias Biológicas y de la Salud
,
Universidad Autónoma Metropolitana
,
2
Medical Research Unit in Biochemistry, Specialties Hospital, National Medical Center SXXI
,
Instituto Mexicano de Seguro Social
,
3
Department of Agricultural and Animal Production, Division of Biological and Health Science
,
Universidad Autónoma Metropolitana-Xochimilco
,
4
Molecular Biology Research Laboratory, Center for Congenital Malformations
,
Children Hospital of Mexico Federico Gomez (HIMFG)
Tags
Keywords: Murine Adipose-derived Mesenchymal Cells
Collagenase
Stromal Vascular Fraction
DMEM
Trypsin EDTA
Hematoxylin And Eosin Staining
Cell Isolation
Cell Maintenance
Cell Characterization
check_url/de/200083?article_type=t
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Murine Adipose-Derived Mesenchymal Cell Isolation, Maintenance and Characterization.
J. Vis. Exp.
(Pending Publication), e200083, doi:10.3791/200083 (2023).
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