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Krebsforschung
Fresh Primary Tumor Tissue Processing to Establish Tumor Organoids and Primary Fibroblasts (Video) | JoVE
Published: May 26, 2023
doi:
10.3791/200204
Jesús Frutos Díaz-Alejo*
1,2,3,4
,
Simon April-Monn*
5
,
Marina Cihova
,
Verona Buocikova
,
Jorge Villalón López
3
,
Maria Urbanova
,
Carmen G. Lechuga
,
Miroslav Tomas
8
,
Peter Dubovan
8
,
Bárbara Luna Sánchez
,
Sonia Camaño Páez
,
Alfonso Sanjuanbenito
9
,
Eduardo Lobo
,
Estefanía Romio de la Heras
,
Carmen Guerra
7
,
Carolina de la Pinta
,
Emma Barreto Melian
2
,
Mercedes Rodríguez Garrote
2
,
Alfredo Carrato
2,4
,
Laura Ruiz-Cañas
12,13
,
Bruno Sainz, Jr.
3,12,13
,
Ana Torres
,
Bozena Smolkova
,
Julie Earl
2,3
1
Molecular Epidemiology and Predictive Tumor Markers Group, Area 3
,
Ramón y Cajal Health Research Institute (IRYCIS)
,
2
The Biomedical Research Network in Cancer (CIBERONC)
,
3
Biobank and Biomodels Platform (PT20/0045), ISCIII research and development platforms in biomedicine and health sciences, BioBank Hospital Ramón y Cajal-IRYCIS, Spanish National Biobanks Network (ISCIII Biobank Register No. B.0000678)
,
Ramón y Cajal Health Research Institute (IRYCIS)
,
4
Faculty of Medicine
,
University of Alcalá de Henares
,
5
Institute of Tissue Medicine and Pathology
,
University of Bern
,
6
Department of Molecular Oncology, Cancer Research Institute
,
Biomedical Research Center of the Slovak Academy of Sciences
,
7
Experimental Oncology, Molecular Oncology Program
,
Centro Nacional de Investigaciones Oncológicas (CNIO)
,
8
Department of Surgical Oncology, National Cancer Institute
,
Slovak Medical University
,
9
Pancreatic and Biliopancreatic Surgery Unit
,
Hospital Universitario Ramón y Cajal
,
10
Department of Pathology
,
Hospital Universitario Ramón y Cajal
,
11
Department of Radiation Oncology
,
Hospital Universitario Ramón y Cajal
,
12
Department of Cancer
,
Instituto de Investigaciones Biomédicas “Alberto Sols” (IIBM)
,
13
Cancer Stem Cell and Fibroinflammatory Group, Chronic Diseases and Cancer, Area 3
,
IRYCIS
Tags
Keywords: Tumor Organoids
Primary Fibroblasts
DMEM/F-12 Media
PBS
Digestion Media
Trypsin
Cell Filtration
Cell Centrifugation
Red Blood Cell Lysis
Cell Dissociation
Cell Resuspension
Membrane Matrix
check_url/de/200204?article_type=t
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Fresh Primary Tumor Tissue Processing to Establish Tumor Organoids and Primary Fibroblasts.
J. Vis. Exp.
(Pending Publication), e200204, doi:10.3791/200204 (2023).
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