Imaging SH-SY5Y Cells by STED Microscopy and Subsequent Analysis of Mitochondrial Ultrastructure

doi:10.3791/200327

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Imaging SH-SY5Y Cells by STED Microscopy and Subsequent Analysis of Mitochondrial Ultrastructure (Video) | JoVE

Published: June 30, 2023

doi:

10.3791/200327

Emery L. Ng, Ashley L. Reed, Christopher B. O’Connell, Nathan N. Alder

¹Center for Open Research Resources and Equipment,University of Connecticut, ²Department of Molecular and Cell Biology,University of Connecticut

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STED Microscopy Mitochondrial Ultrastructure SH-SY5Y Cells LightBox Software Confocal Acquisition Settings STED Acquisition Deconvolution Trainable Weka Segmentation Cristae Image Analysis

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Imaging SH-SY5Y Cells by STED Microscopy and Subsequent Analysis of Mitochondrial Ultrastructure. J. Vis. Exp. (Pending Publication), e200327, doi:10.3791/200327 (2023).

Imaging SH-SY5Y Cells by STED Microscopy and Subsequent Analysis of Mitochondrial Ultrastructure (Video) | JoVE

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