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Biologie
Sample Preparation and Fluorescence Measurement of Stained Transfected HEK293T Cells (Video) | JoVE
Published: June 02, 2023
doi:
10.3791/200360
Tomer Eli Ben Yosef
,
Raz Zarivach
,
Arie Moran
1
Department of Life Sciences and National Institute for Biotechnology in the Negev
,
Ben-Gurion University
,
2
Department of Physiology and Cell Biology, Faculty of Health Sciences
,
Ben-Gurion University
Tags
Keywords: Sample Preparation
Fluorescence Measurement
Transfected HEK293T Cells
Microscope
Light Source
Camera
Suction System
Ringer’s Solution
Ringer’s Zinc Solution
Pyrithione
Perfusion Chamber
Sealing Silicone
Coverslip
Ringer’s Washing Solution
Perfusion Rate
Imaging Software
MCherry
ROIs
Background ROI
GFP
Perfect Focus System
PFS
check_url/de/200360?article_type=t
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Sample Preparation and Fluorescence Measurement of Stained Transfected HEK293T Cells.
J. Vis. Exp.
(Pending Publication), e200360, doi:10.3791/200360 (2023).
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