Separation and Analysis of Submitochondrial Vesicles to Identify Mitochondrial Contact Site Proteins

Published: June 16, 2023

doi:

10.3791/200432

Siavash Khosravi*^1,2, Johanna Frickel*¹, Max E. Harner

¹Department of Cell Biology, Biomedical Center, Medical Faculty,Ludwig-Maximilians University Munich, ²Cardio-Metabolic Diseases,Boehringer Ingelheim Pharma GmbH & Co. KG

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Keywords: – Submitochondrial Vesicles – Mitochondrial Contact Site Proteins – Centrifugation – Sucrose Cushion – Sucrose Gradient – Trichloroacetic Acid Precipitation – Protein Analysis – Mitochondria – Yeast – Homogenization – Refractometer – MOPS Buffer – EDTA – PMSF – Protease Inhibitors

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Separation and Analysis of Submitochondrial Vesicles to Identify Mitochondrial Contact Site Proteins. J. Vis. Exp. (Pending Publication), e200432, doi:10.3791/200432 (2023).

Separation and Analysis of Submitochondrial Vesicles to Identify Mitochondrial Contact Site Proteins

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