JoVE Journal > Cancer Research

Lentiviral Packaging and Viral Titering for Genome-Scale shRNA Screens (Video) | JoVE

Published: August 25, 2023

doi:

Brittany Turner-Ivey, Jody Fromm Longo, Dorea P. Jenkins, Stephen T. Guest, Steven L. Carroll²

¹Hollings Cancer Center,Medical University of South Carolina, ²Department of Pathology and Laboratory Medicine,Medical University of South Carolina, ³Department of Computational Medicine and Bioinformatics,University of Michigan, Ann Arbor

Tags

Lentiviral Packaging Viral Titering Genome-scale ShRNA Screens 293T Cells Packaging Plasmid Mix Plasmid Barcode Library Transfection Reagent Viral Particles Viral Titering Cationic Polymer Tumor Cell Growth Media Tumor Cells Lentivirus

Play Video

DOI

Diesen Artikel zitieren

Lentiviral Packaging and Viral Titering for Genome-Scale shRNA Screens. J. Vis. Exp. (Pending Publication), e200477, doi:10.3791/200477 (2023).

View Video