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Biologie
Flow Cytometric Analysis of Mitophagy in Murine Pancreatic β-Cells Using MtPhagy Dye (Video) | JoVE
Published: September 15, 2023
doi:
10.3791/200661
Elena Levi-D’Ancona
2
,
Vaibhav Sidarala
,
Scott A. Soleimanpour
3,4
1
Department of Internal Medicine, Division of Metabolism, Endocrinology and Diabetes
,
University of Michigan, Ann Arbor
,
2
Graduate Program in Immunology
,
University of Michigan Medical School
,
3
Department of Molecular and Integrative Physiology
,
University of Michigan, Ann Arbor
,
4
VA Ann Arbor Healthcare System
Tags
Keywords: Flow Cytometry
Mitophagy
Pancreatic Beta Cells
MtPhagy Dye
TMRE
Fluozin-3-AM
DAPI
Cell Suspension
Islet Isolation
Fluorescence Gating
Quantification
check_url/de/200661?article_type=t
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Flow Cytometric Analysis of Mitophagy in Murine Pancreatic β-Cells Using MtPhagy Dye.
J. Vis. Exp.
(Pending Publication), e200661, doi:10.3791/200661 (2023).
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