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Neuroscience
Constructing the 3D Human-Stem-Cell-Derived In Vitro Blood Brain Barrier (Video) | JoVE
Published: October 20, 2023
doi:
10.3791/200668
Camille Goldman*
1,2,3,4,5
,
Natalie Suhy*
1,2,3,4,5
,
Jessica E. Schwarz
2,3,4,5
,
Emily Ruth Sartori
2,3,4,5
,
Rikki B. Rooklin
2,3,4,5
,
Braxton R. Schuldt
2,3,4,5
,
Louise A. Mesentier-Louro
2,3,4,5
,
Joel W. Blanchard
2,3,4,5
1
Icahn School of Medicine at Mount Sinai
,
2
Black Family Stem Cell Institute at Mount Sinai
,
3
Ronald M. Loeb Center for Alzheimer’s Disease at Mount Sinai
,
4
Friedman Brain Institute at Mount Sinai
,
5
Nash Family Department of Neuroscience at Mount Sinai
Tags
Keywords: 3D Human-stem-cell-derived
In Vitro Blood-brain Barrier
IBBB
Dissociated Endothelial Cells
Pericytes
Astrocytes
Cell Mixture
Cell Pellet
Reduced GF Basement Membrane Matrix
Cell Matrix Mixture
48-well Or 96-well Glass-bottom Culture Dish
Polymerize The Matrix
Supplemented Astrocyte Medium
96-well Plate
Downstream Assays
Properly Formed IBBB
Single Translucent Disk
Single Cells
check_url/de/200668?article_type=t
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Cite This Article
Constructing the 3D Human-Stem-Cell-Derived In Vitro Blood Brain Barrier.
J. Vis. Exp.
(Pending Publication), e200668, doi:10.3791/200668 (2023).
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