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Medizin
Isolation of Limbal Niche Cells (LNCs) from Limbus Tissue (Video) | JoVE
Published: October 27, 2023
doi:
10.3791/200727
Guanyu Su
,
Wei Wang
,
Lingjuan Xu
,
Rong Liu
,
Yongyao Tan
,
Bihui Jin
,
Chunxiu You
,
Xiao Zhou
,
Yihong Xiong
,
Huatao Xie
,
Guigang Li
1
Department of Ophthalmology, Tongji Hospital, Tongji Medical College
,
Huazhong University of Science and Technology
,
2
Institute of Medicine Nursing
,
Hubei University of Medicine
,
3
Department of Ophthalmology, Union Hospital, Tongji Medical College
,
Huazhong University of Science and Technology
Tags
Limbal Niche Cells
LNCs
Collagenase A
Matrigel
Basement Membrane Matrix
MESCM
Trypsin-EDTA
Knockout Serum
Cell Isolation
Cell Culture
Cornea
Sterile Technique
check_url/de/200727?article_type=t
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Isolation of Limbal Niche Cells (LNCs) from Limbus Tissue.
J. Vis. Exp.
(Pending Publication), e200727, doi:10.3791/200727 (2023).
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