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Biochemie
Reductive Methylation and Pre-Crystallization Treatment of Purified Heterodimeric ABCG5/G8 Protein (Video) | JoVE
Published: August 25, 2023
doi:
10.3791/200736
Sarah Wazir
,
Danny Farhat
,
Mahalashmi Srinivasan
2
,
Jyh-Yeuan Lee
1
Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine
,
University of Ottawa
,
2
Department of Biochemistry, Faculty of Medicine and Dentistry
,
University of Alberta
Tags
Keywords: Reductive Methylation
ABCG5/G8 Protein
Purification
Pre-crystallization Treatment
Nickel-NTA Column
PD-10 Desalting
Cholesterol Addition
Ultracentrifugation
Protein Concentration
Analytical Gel Filtration
check_url/de/200736?article_type=t
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Reductive Methylation and Pre-Crystallization Treatment of Purified Heterodimeric ABCG5/G8 Protein.
J. Vis. Exp.
(Pending Publication), e200736, doi:10.3791/200736 (2023).
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