SARS-CoV-2 Pseudotype Virus Production and Serum Neutralization Assay - JoVE

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SARS-CoV-2 Pseudotype Virus Production and Serum Neutralization Assay (Video) | JoVE

Published: November 21, 2023

doi:

Tobia Fantoni*¹, Michele Bissoli*¹, Chiara Stefani, Mauro Voi, Alexandrina Dabija, Rebecca Casula, Domenico Luca Minafra, Julys da Fonseca Palmeira, Enrique Roberto Argañaraz, Martin Mayora-Neto, Nigel J. Temperton, Donato Zipeto, Alessandra Ruggiero

¹Department of Neuroscience, Biomedicine and Movement,University of Verona, ²Laboratory of Molecular Neurovirology, Faculty of Health Science,University of Brasília, ³Viral Pseudotype Unit, Medway School of Pharmacy,Universities of Kent and Greenwich at Medway

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Keywords: SARS-CoV-2 Pseudotype Virus Serum Neutralization Assay HEK 293T Cells Transfection Polyethylenimine Cell Culture Virus Production Viral Supernatant Serial Dilution HEK 293T ACE2 Cells Luciferase Assay Serum Samples Heat Inactivation

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Diesen Artikel zitieren

SARS-CoV-2 Pseudotype Virus Production and Serum Neutralization Assay. J. Vis. Exp. (Pending Publication), e200835, doi:10.3791/200835 (2023).

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