Chromosome Preparation and Karyotype Analysis of CRISPR/Cas9-Mediated CENP-E Knockout HeLa Cells - JoVE

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Chromosome Preparation and Karyotype Analysis of CRISPR/Cas9-Mediated CENP-E Knockout HeLa Cells

Published: June 23, 2023

doi:

Meng-Fei Xu², Jie Chen², Yue Xu², Jing-Lian Zhang², Yi Zhou², Jie-Jie He², Shan Wu², Ya-Lan Wei⁴, Zhen-Yu She²

¹Department of Cell Biology and Genetics, The School of Basic Medical Sciences,Fujian Medical University, ²Key Laboratory of Stem Cell Engineering and Regenerative Medicine,Fujian Province University, ³College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics,Fujian Medical University, ⁴Medical Research Center,Fujian Maternity and Child Health Hospital

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Keyword Extraction: Chromosome Preparation Karyotype Analysis CRISPR/Cas9 CENP-E Knockout HeLa Cells Colchicine Trypsin-EDTA Potassium Chloride Fixative Solution Giemsa’s Staining Light Microscope Plan Fluor Objective Chromosome Number Wild Type Mutant

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Chromosome Preparation and Karyotype Analysis of CRISPR/Cas9-Mediated CENP-E Knockout HeLa Cells. J. Vis. Exp. (Pending Publication), e201004, doi:10.3791/201004 (2023).

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