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JoVE Journal > Biochemistry
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Biochemie
Click Assay for the Measurement of Histone Acetyltransferase 1 Acetylation
Published: January 26, 2024
doi:
10.3791/201342
Shreenidhi Rajkumar
,
Danielle Dixon
,
Andrew M. Lipchik
,
Joshua J. Gruber
1
Departments of Internal Medicine and the Green Center for Reproductive Biology Sciences
,
University of Texas Southwestern Medical Center
,
2
Department of Pharmaceutical Sciences, Eugene Applebaum College of Pharmacy and Health Sciences
,
Wayne State University
Tags
Keywords: Click Assay
Histone Acetyltransferase 1 Acetylation
Biotinylated Histone H4 Peptide
Acetylation Reactions
4-Penten Oil Co-enzyme A
BSA Pre-blocked Black NeutrAvidin-coated 96-well Plates
THPTA Copper Mixture
Sodium Ascorbate
Biotin Azide
Streptavidin Horseradish Peroxidase
Amplex Red Detection Reagents
Hydrogen Peroxide
Sodium Phosphate
check_url/de/201342?article_type=t
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Click Assay for the Measurement of Histone Acetyltransferase 1 Acetylation.
J. Vis. Exp.
(Pending Publication), e201342, doi:10.3791/201342 (2024).
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