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JoVE Journal > Immunology and Infection
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Immunologie und Infektion
Preparation and Purification of T4 Phage Lysates for Oral Administration in Mice
Published: January 26, 2024
doi:
10.3791/201384
Nicola Pett
,
Michael Hunter
,
Natalia A. Carranza García
,
Jung Hee Seo
,
Samuel R. Collins
,
Forest Rohwer
,
Lisa C. Osborne
,
Carolina Tropini
3,4
1
Department of Microbiology and Immunology, Life Sciences Institute
,
University of British Columbia
,
2
Department of Biology
,
San Diego State University
,
3
School of Biomedical Engineering
,
University of British Columbia
,
4
Humans and the Microbiome Program
,
Canadian Institute for Advanced Research
Tags
Keyword Extraction: T4 Phage
Escherichia Coli
Luria-Bertani (LB) Medium
Phage Lysates
Oral Administration
Centrifugal Filtration
Endotoxin Removal
Saline Magnesium (SM) Buffer
Chloroform Treatment
Bacterial Culture
Bacterial Debris Removal
check_url/de/201384?article_type=t
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Preparation and Purification of T4 Phage Lysates for Oral Administration in Mice.
J. Vis. Exp.
(Pending Publication), e201384, doi:10.3791/201384 (2024).
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