Source: Murphy, K. A. et al. A Syngeneic Mouse Model of Metastatic Renal Cell Carcinoma for Quantitative and Longitudinal Assessment of Preclinical Therapies. J. Vis. Exp. (2017)
In this video, we develop an orthotopic mouse model of renal cell carcinoma. This model results in reproducible primary tumors in the injected kidney and metastatic tumors in the lung, allowing the evaluation of disease progression and treatment.
1. Implantation of Cells
To implant Renca cells intrarenally, prepare Renca cells into a single-cell suspension at 2 x 106 cells/mL in Hank's Balanced Salt Solution (HBSS).
2. Surgical Area Preparation
3. Animal Preparation
The authors have nothing to disclose.
Artificial tears ophthalmic ointment | Akorn | NDC 17478-162-35 | |
Vetbond Tissue Adhesive | 3M | CBGBIW011019 | |
Betadine Solution (Povidoneiodine, 5%) NDC | Purdue Products, L.P. | 67618-155-32 | |
0.25% Marcaine (Bupivavaine HCl injection) | Hospira | 0409-1587-50 | |
Heating pad | Sunbeam | ||
Syringes BD | 309659 | ||
Gauze | Venture | 908291 | |
Ketamine | Phoenix Pharmaceuticals NDC | 57319-609-02 | |
Xylazine | Akorn NDC | 59399-111 | |
Hank’s Balanced Salts | Sigma-Aldrich | H2387 | |
Scissors | |||
Forceps | |||
Renca | ATCC | CRL-2947 |