Source: Hatami, S., et al. Normothermic Ex Situ Heart Perfusion in Working Mode: Assessment of Cardiac Function and Metabolism. J. Vis. Exp. (2019).
In this video, we describe a step-by-step protocol to extract the heart from a porcine model for cardiac transplantation.
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Blood Collection and Heart Procurement
The authors have nothing to disclose.
Debakey-Metzenbaum dissecting scissors | Pilling | 342202 | |
MAYO dissecting scissors | Pilling | 460420 | |
THUMB forceps | Pilling | 465165 | |
Debakey straight vascular tissue forceps | Pilling | 351808 | |
CUSHING Gutschdressing forceps | Pilling | 466200 | |
JOHNSON needle holder | Pilling | 510312 | |
DERF needle holder | Pilling | 443120 | |
Sternal saw | Stryker | 6207 | |
Sternal retractor | Pilling | 341162 | |
Vorse tubing clamp | Pilling | 351377 | |
MORRIS ascending aorta clamp | Pilling | 353617 | |
Surgical snare (tourniquet) set | Medtronic | CVR79013 | |
2-0 SILK black 12" x 18" strands | ETHICON | A185H | |
3-0 PROLENE blue 18" PS-2 cutting | ETHICON | 8687H | |
DLP cardioplegia cannula | Medtronics | 20613994495406 | Aortic root cannula |
5F Ventriculr straight pigtail cathter | CORDIS | 534550S | |
5F AVANTI+ Sheath Introducer | CORDIS | 504605A | |
Surgical electrocautery device Kls | Martin | ME411 |