Simple Establishment of a Vascularized Osteogenic Bone Marrow Niche Using Pre-Cast Poly(ethylene Glycol) (PEG) Hydrogels in an Imaging Microplate

Lisa  A. Krattiger; Maria Mitsi; Benjamin R. Simona; Martin Ehrbar

doi:10.3791/65413

3D Co-Culture Assay: Vascularized Osteogenic Niches for Cancer Drug Screening

May 19, 2023

Simple Establishment of a Vascularized Osteogenic Bone Marrow Niche Using Pre-Cast Poly(Ethylene Glycol) (PEG) Hydrogels in an Imaging Microplate (Video) | JoVE

01:15 min

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Establishing the 3D Stromal Monoculture (Video) | JoVE

01:46 min

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Establishing the 3D Stromal-Endothelial Cell Co-Culture (Video) | JoVE

01:48 min

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Quantifying the Endothelial Cell Network Formation After the 3D Stromal-Endothelial Cell Co-Culture (Video) | JoVE

02:34 min

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Assessing the Osteogenic Differentiation of 3D Stromal-Endothelial Cells by Monitoring the Alkaline Phosphatase Activity (Video) | JoVE

03:44 min

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Lisa A. Krattiger, Maria Mitsi, Benjamin R. Simona, Martin Ehrbar

¹Department of Obstetrics,University Hospital Zurich, University of Zurich, ²Ectica Technologies AG

Summary

An in vitro model of the bone marrow vascular niche is established by seeding mesenchymal and endothelial cells onto pre-cast 3D PEG hydrogels. The endothelial networks, ECM components, and ALP activity of the niches vary depending on the growth factor used. The platform can be used for advanced cancer models.