1Department of Cardiovascular Surgery, West China Hospital,Sichuan University, 2Department of Integrated Traditional Chinese and Western Medicine, Sichuan Provincial Pancreatitis Centre, West China Hospital,Sichuan University, 3West China-Liverpool Biomedical Research Centre, West China Hospital,Sichuan University, 4Cardiovascular Surgery Research Laboratory, West China Hospital,Sichuan University
This research outlines two techniques for isolating abundant neutrophil extracellular traps (NETs) from rat bone marrow. One method combines a commercial neutrophil isolation kit with density gradient centrifugation, while the other employs only density gradient centrifugation. Both approaches yield functional NETs surpassing those from peripheral blood neutrophils.
