Coating TEM Grids with a Graphene Monolayer

September 08, 2023

Author Spotlight: Enhancing CryoEM Resolution Using Graphene-Coated Grids

Coating Single-Layer Graphene-Copper Sheet with MMA and Cleaning the Grids

Transferring Grids to a Perforated Tray Under DI Water, Transferring Graphene to the Grids, Removing MMA, and Cleaning the Grids

Benjamin Basanta*¹, Wenqian Chen*¹, Daniel E. Pride, Gabriel C. Lander

¹Department of Structural and Computational Biology,Scripps Research

Summary

Here, we describe a protocol for applying a single monolayer of graphene to electron microscopy grids and how to prepare them for use in cryoEM structure determination.

Article