Purification of Porcine Islets by Surgical Perfusion of Pig Pancreas

June 07, 2024

Author Spotlight: Techniques and Challenges in Islet Purification for Xenotransplantation

Sophia H. Roberts², Louai Alrata², Dahlia Abdulsattar², Karan Joseph², Wahid Abu-Amer², Mohamed Zaghloul², Batool Arif², Tarek Alhamad, Maria S. Remedi, Yiing Lin, Mohamed A. Zayed^2,6,7

¹Department of Surgery, Section of Vascular Surgery,Washington University School of Medicine, ²CardioVascular Research Innovation in Surgery and Engineering Center,Washington University School of Medicine, ³Department of Medicine, Division of Nephrology,Washington University School of Medicine, ⁴Department of Medicine, Division of Endocrinology,Washington University School of Medicine, ⁵Department of Surgery, Section of Transplant Surgery,Washington University School of Medicine, ⁶Division of Molecular Cell Biology,Washington University School of Medicine, ⁷Department of Biomedical Engineering,Washington University McKelvey School of Engineering

Summary

This protocol provides a step-by-step guide for the procurement of a porcine pancreas for islet isolation and purification.

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