China Medical University Hospital 4 articles published in JoVE Medicine Efficacy of Fu's Subcutaneous Needling on Sciatic Nerve Pain: Behavioral and Electrophysiological Changes in a Chronic Constriction Injury Rat Model Po-En Chiu*1,2, Zhonghua Fu*3,4, De-Wei Lai5,6, Li-Wei Chou7,8,9 1Department of Chinese Medicine, Chang Bing Show Chwan Memorial Hospital, 2Graduate Institute of Integrated Medicine, College of Chinese Medicine, China Medical University, 3Institute of Fu's Subcutaneous Needling, Beijing University of Chinese Medicine, 4Clinical Medical College of Acupuncture & Moxibustion and Rehabilitation, Guangzhou University of Chinese Medicine, 5Experimental Animal Center, Department of Molecular Biology and Cell Research, Chang Bing Show Chwan Memorial Hospital, 6Department of Pharmacy and Master Program, Tajen University, 7Department of Physical Medicine and Rehabilitation, China Medical University Hospital, 8Department of Physical Therapy and Graduate Institute of Rehabilitation Science, China Medical University, 9Department of Physical Medicine and Rehabilitation, Asia University Hospital, Asia University We present a protocol for using Fu's subcutaneous needling in a chronic constriction injury model to induce sciatic nerve pain in rats. Medicine Fu's Subcutaneous Needling for Knee Osteoarthritis Pain Po-En Chiu*1,2, Zhonghua Fu*3,4, Jian Sun4,5,6, Guan-Wei Jian7,8, Te-Mao Li9, Li-Wei Chou10,11,12 1Department of Chinese Medicine, Chang Bing Show Chwan Memorial Hospital, 2Graduate Institute of Integrated Medicine, College of Chinese Medicine, China Medical University, 3Institute of Fu's Subcutaneous Needling, Beijing University of Chinese Medicine, 4Clinical Medical College of Acupuncture & Moxibustion and Rehabilitation, Guangzhou University of Chinese Medicine, 5Second Clinical Medical College, Guangzhou University of Chinese Medicine, 6Guangdong Provincial Hospital of Chinese Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 7Graduate Institute of Acupuncture Science, China Medical University, 8Department of Chinese Medicine, Sinying Hospital, Ministry of Health and Welfare, 9School of Chinese Medicine, College of Chinese Medicine, China Medical University, 10Department of Physical Medicine and Rehabilitation, China Medical University Hospital, 11Department of Physical Therapy and Graduate Institute of Rehabilitation Science, China Medical University, 12Department of Physical Medicine and Rehabilitation, Asia University Hospital, Asia University We present a protocol for using Fu's subcutaneous needling for knee osteoarthritis pain, which combines swaying movement and reperfusion approach techniques. This protocol has great potential for future applications in myofascial pain treatment and could enhance Fu's subcutaneous needling (FSN) manipulation skills. Cancer Research Isolation and Expansion of Cytotoxic Cytokine-induced Killer T Cells for Cancer Treatment Chi-Hao Hsiao1,2,3, Ya-Hsu Chiu4, Shao-Chih Chiu4,5, Der-Yang Cho4,6,7, Liang-Ming Lee1,2, Yu-Ching Wen1,2, Jia-Jhe Li4, Ping-Hsiao Shih4 1Department of Urology, Wan Fang Hospital, 2Department of Urology, School of Medicine, College of Medicine, Taipei Medical University, 3Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, 4Translational Cell Therapy Center, Department of Medical Research, China Medical University Hospital, 5Graduate Institute of Biomedical Sciences, China Medical University, 6Graduate Institute of Immunology, China Medical University, 7Department of Neurosurgery, Neuropsychiatric Center, China Medical University Hospital Here, we present a protocol to perform the isolation and expansion of peripheral blood mononuclear cells-derived cytokine-induced CD3+CD56+ killer cells and illustrate their cytotoxicity effect against hematological and solid cancer cells by using an in vitro diagnosis flow cytometry system. Medicine Simultaneous Electrocardiography Recording and Invasive Blood Pressure Measurement in Rats Hsin-Ting Lin1,2, Yi-Lin Shiou1, Wun-Jyun Jhuang1, Hsiang-Chun Lee1,3,4,5,6 1Division of Cardiology, Department of Internal Medicine, Kaohsiung Medical University Hospital, 2Hematology and Oncology, China Medical University Hospital, 3Department of Internal Medicine, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, 4Center for Lipid Biosciences, Kaohsiung Medical University Hospital, 5Lipid Science and Aging Research Center, Kaohsiung Medical University, 6Institute/Center of Medical Science and Technology, National Sun Yat-sen University Here, we describe a setup for simultaneous recording of electrocardiography and intra-arterial blood pressure (BP) in experimental rats, which can be done with standard equipment in animal facilities and can be applied to physiological or pharmacological studies to investigate pathogenic or therapeutic mechanisms in cardiovascular medicine.
