University of Palermo 3 articles published in JoVE Engineering Generation and Coherent Control of Pulsed Quantum Frequency Combs Benjamin MacLellan*1, Piotr Roztocki*1, Michael Kues1,2, Christian Reimer1, Luis Romero Cortés1, Yanbing Zhang1, Stefania Sciara1,3, Benjamin Wetzel1,4, Alfonso Cino3, Sai T. Chu5, Brent E. Little6, David J. Moss7, Lucia Caspani8, José Azaña1, Roberto Morandotti1,9,10 1Institut National de la Recherche Scientifique - Centre Énergie, Matériaux et Télécommunications (INRS-EMT), 2School of Engineering, University of Glasgow, 3Department of Energy, Information Engineering and Mathematical Models, University of Palermo, 4School of Mathematical and Physical Sciences, University of Sussex, 5Department of Physics and Material Science, City University of Hong Kong, 6State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Science, 7Centre for Micro Photonics, Swinburne University of Technology, 8Institute of Photonics, Department of Physics, University of Strathclyde, 9Institute of Fundamental and Frontier Sciences, University of Electronic Science and Technology of China, 10National Research University of Information Technologies, Mechanics and Optics A protocol is presented for the practical generation and coherent manipulation of high-dimensional frequency-bin entangled photon states using integrated micro-cavities and standard telecommunications components, respectively. Bioengineering A Facile and Eco-friendly Route to Fabricate Poly(Lactic Acid) Scaffolds with Graded Pore Size Roberto Scaffaro1, Francesco Lopresti1, Luigi Botta1, Andrea Maio1, Fiorenza Sutera1, Maria Chiara Mistretta1, Francesco Paolo La Mantia1 1Department of civil, environmental, aerospace, and materials engineering (DICAM), RU INSTM, University of Palermo In this work, poly(lactic acid)/polyethylene glycol (PLA/PEG) scaffolds were prepared by using a combination of melt mixing and selective leaching. The method herein discussed permitted to develop three-layer scaffolds by highly controlling both porosity and pore size. The mechanical properties were also evaluated in a physiological environment. Medicine Exosomal miRNA Analysis in Non-small Cell Lung Cancer (NSCLC) Patients' Plasma Through qPCR: A Feasible Liquid Biopsy Tool Marco Giallombardo*1,2, Jorge Chacártegui Borrás*2,3, Marta Castiglia4, Nele Van Der Steen3, Inge Mertens5,6, Patrick Pauwels7,3, Marc Peeters8,3, Christian Rolfo2,3 1Department of Biopathology and Medical Biotechnology, Section of Biology and Genetics, University of Palermo, 2Phase I-Early Clinical Trials Unit, Oncology Department, Antwerp University Hospital (UZA), 3Center for Oncological Research (CORE), Antwerp University, 4Department of Surgical, Oncological and Oral Sciences, Section of Medical Oncology, University of Palermo, 5Flemish Institute for Technological Research (VITO), 6CORE, Campus Groenenborger, Antwerp University, 7Molecular Pathology, Pathology Department, Antwerp University Hospital (UZA), 8Oncology Department, Antwerp University Hospital (UZA) This protocol describes the feasibility to perform miRNA profiling in exosomes, released in plasma of NSCLC patients, through a commercial exosome isolation kit with Proteinase K and RNAse treatments, in order to avoid circulating miRNAs contamination and evaluate their biomarker features in NSCLC.