Universidade Federal do ABC 1 article published in JoVE Neuroscience An Experiment Using Functional Near-Infrared Spectroscopy and Robot-Assisted Multi-Joint Pointing Movements of the Lower Limb João Ricardo Sato1, Anna Carolyna Gianlorenço2, Elayne Borges Fernandes2, Thalita Frigo da Rocha2, Antonio Massato Makiyama*3, Laura Dipietro*4 1Center of Mathematics, Computing and Cognition, Universidade Federal do ABC, 2Department of the Physical Therapy, Federal University of Sao Carlos, 3Vivax Ltda, 4Highland Instruments It is estimated that 1 in 6 individuals worldwide will have a stroke in their lifetime, causing long-term disability, whose rehabilitation mechanisms are still poorly understood. This study proposes a protocol to evaluate brain activation by functional near-infrared spectroscopy (fNIRS) during a lower limb robotic therapy session.