JOVE JOURNAL

Biochemie

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A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1

Eric D. Routh*¹, Steven D. Creacy*², Peter E. Beerbower, Steven A. Akman, James P. Vaughn, Philip J. Smaldino

¹Department of Cancer Biology,Wake Forest School of Medicine, ²YX Genomics, ³Department of Biology,Ball State University, ⁴Department of Hematology and Oncology,Roper St. Francis Hospital

Journal (Biochemistry)

Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy

Ramakrishnan B. Kumar, Lin Zhu, Hans Hebert², Caroline Jegerschöld²

¹Department of Biosciences and Nutrition,Karolinska Institutet, ²School of Technology and Health,KTH Royal Institute of Technology

Journal (Biochemistry)

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

Robyn M. Johnston, Anne W. Sylvester, Michael J. Scanlon

¹Plant Biology Section, School of Integrative Plant Science,Cornell University, ²Department of Developmental Genetics,University of Wyoming

Journal (Biochemistry)

Differential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen

Ibrahim B. Durowoju, Kamaljit S. Bhandal, Jian Hu, Bruce Carpick, Marina Kirkitadze

¹Analytical Research & Development,Sanofi Pasteur Limited

Journal (Biochemistry)

Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues

Lisa M. Meints, Anne W. Poston, Brent F. Piligian, Claire D. Olson, Katherine S. Badger, Peter J. Woodruff, Benjamin M. Swarts

¹Department of Chemistry and Biochemistry,Central Michigan University, ²Department of Chemistry,University of Southern Maine

Journal (Biochemistry)

Structural Information from Single-molecule FRET Experiments Using the Fast Nano-positioning System

Thilo Dörfler*¹, Tobias Eilert*¹, Carlheinz Röcker, Julia Nagy, Jens Michaelis

¹Institute of Biophysics,Ulm University

Journal (Biochemistry)

A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing

Irena Roci², Hector Gallart-Ayala, Jeramie Watrous, Mohit Jain, Craig E. Wheelock, Roland Nilsson²

¹Department of Medicine, Unit of Computational Medicine,Karolinska Institutet, ²Center for Molecular Medicine,Karolinska Institutet, ³Department of Medical Biochemistry and Biophysics, Division of Physiological Chemistry 2,Karolinska Institutet, ⁴Department of Medicine,University of California San Diego

Journal (Biochemistry)

Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells

Zheng Ma*¹, Victor Fung*¹, Iván D'Orso

¹Department of Microbiology,The University of Texas Southwestern Medical Center

Journal (Biochemistry)

Preparation of Giant Vesicles Encapsulating Microspheres by Centrifugation of a Water-in-oil Emulsion

Yuno Natsume, Hsin-i Wen, Tong Zhu, Kazumi Itoh, Li Sheng, Kensuke Kurihara^3,4

¹Department of Mathematical and Physical Sciences, Faculty of Science,Japan Women's University, ²Department of Bioorganization Research, Okazaki Institute for Integrative Bioscience,National Institutes of Natural Sciences, ³Department of Life and Coordination-Complex Molecular Science, Institute for Molecular Science,National Institutes of Natural Sciences, ⁴Research Center for Complex Systems Biology,The University of Tokyo

Journal (Biochemistry)

Laboratory Scale Production and Purification of a Therapeutic Antibody

Zehra Elgundi, Vicki Sifniotis, Mouhamad Reslan, Esteban Cruz, Veysel Kayser

¹Faculty of Pharmacy,University of Sydney

Journal (Biochemistry)

A Protein Preparation Method for the High-throughput Identification of Proteins Interacting with a Nuclear Cofactor Using LC-MS/MS Analysis

Megumi Tsuchiya*¹, M. Rezaul Karim*², Taro Matsumoto, Hidesato Ogawa, Hiroaki Taniguchi^4,5

¹Graduate School of Frontier Biosciences,Osaka University, ²Department of Biotechnology and Genetic Engineering,Jahangirnagar University, ³Division of Cell Regeneration and Transplantation,School of Medicine, Nihon University, ⁴Institute of Genetics and Animal Breeding of the Polish Academy of Sciences, ⁵Graduate School of Life and Medical Sciences,Doshisha University

Journal (Biochemistry)

Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay

Xinzhou Zhu, Andrea Zelmer, Sven Wellmann²

¹University Children's Hospital Basel (UKBB),University of Basel, ²Department of Clinical Research,University of Basel

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