All Protocols and Video Articles for scientific research | JoVE

Showing 49 - 60 of 477 videos for "Reconstitution"

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Journal (Cancer Research)

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Methodensammlungen

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer

Maryam Nakhjavani², Breanna Giles², Mia Strom², Chris Vi², Sahara Attenborough², Sarah Shigdar²

¹School of Medicine,Deakin University, ²Institute for Mental and Physical Health and Clinical Translation, School of Medicine,Deakin University

Journal (Biology)

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Methodensammlungen

Fast Colony Forming Unit Counting in 96-Well Plate Format Applied to the Drosophila Microbiome

Ren Dodge, William B. Ludington²

¹Department of Embryology,Carnegie Institution for Science, ²Department of Biology,Johns Hopkins University

Journal (Bioengineering)

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Methodensammlungen

Reconstitution of Actin-Based Motility with Commercially Available Proteins

Cécile Sykes, Julie Plastino

¹Laboratoire de physique de l’Ecole Normale Supérieure, ENS, Université PSL, CNRS,Sorbonne Université, Université Paris Cité

Journal (Biology)

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Methodensammlungen

Flow Cytometry Analysis of Murine Bone Marrow Hematopoietic Stem and Progenitor Cells and Stromal Niche Cells

Laura Mosteo, Joana Reis, Lídia Rocha, Marta Lopes, Delfim Duarte^2,3,4

¹Instituto de Investigação e Inovação em Saúde (i3S),Universidade do Porto, ²Department of Onco-Hematology,Instituto Português de Oncologia (IPO)-Porto, ³Unit of Biochemistry, Department of Biomedicine,Faculdade de Medicina da Universidade do Porto, ⁴Porto Comprehensive Cancer Center (P.CCC)

Journal (Genetics)

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Methodensammlungen

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

Diedre Reitz, Jérôme Savocco, Aurèle Piazza, Wolf-Dietrich Heyer³

¹Department of Microbiology & Molecular Genetics,University of California, Davis, ²Laboratory of Biology & Modeling of the Cell,École Normale Supérieure de Lyon, ³Department of Molecular & Cellular Biology,University of California, Davis

Journal (Biology)

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Methodensammlungen

Reconstituting and Characterizing Actin-Microtubule Composites with Tunable Motor-Driven Dynamics and Mechanics

Mehrzad Sasanpour, Daisy H. Achiriloaie², Gloria Lee, Gregor Leech, Maya Hendija, K. Alice Lindsay, Jennifer L. Ross, Ryan J. McGorty, Rae M. Robertson-Anderson

¹Department of Physics and Biophysics,University of San Diego, ²W. M. Keck Science Department,Scripps College, Pitzer College, and Claremont McKenna College, ³Department of Physics,Syracuse University

Journal (Biochemistry)

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Methodensammlungen

Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection

Jonathan T. Specker, Alexander B. Smith, Orlaith Keenan, Joseph P. Zackular³, Boone M. Prentice

¹Department of Chemistry,University of Florida, ²Division of Protective Immunity,Children’s Hospital of Philadelphia, ³Department of Pathology and Laboratory Medicine, Perelman School of Medicine,University of Pennsylvania

Journal (Biochemistry)

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Methodensammlungen

Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions

Brandy N. Curtis*¹, Ellysa J. D. Vogt*², Kevin S. Cannon³, Amy S. Gladfelter^2,3

¹Department of Biochemistry and Biophysics,University of North Carolina at Chapel Hill, ²Curriculum in Genetics and Molecular Biology,University of North Carolina at Chapel Hill, ³Department of Biology,University of North Carolina at Chapel Hill

Journal (Neuroscience)

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Methodensammlungen

Postural Organization of Gait Initiation for Biomechanical Analysis Using Force Platform Recordings

Arnaud Simonet^2,3, Arnaud Delafontaine^3,4, Paul Fourcade³, Eric Yiou³

¹LADAPT Loiret,Centre de Soins de Suite et de Réadaptation, ²CIAMS,Université Paris-Saclay, ³CIAMS,Université d'Orléans, ⁴Université Libre de Bruxelles, Belgique

Journal (Biochemistry)

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Methodensammlungen

Visualizing Actin and Microtubule Coupling Dynamics In Vitro by Total Internal Reflection Fluorescence (TIRF) Microscopy

Jessica L. Henty-Ridilla

¹Department of Biochemistry & Molecular Biology, and Department of Neuroscience & Physiology,State University of New York (SUNY) Upstate Medical University

Journal (Biology)

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Methodensammlungen

CRISPR/Cas9 Gene Editing of Hematopoietic Stem and Progenitor Cells for Gene Therapy Applications

Vigneshwaran Venkatesan*^1,2, Abisha Crystal Christopher*^1,3, Karthik V. Karuppusamy², Prathibha Babu², Manoj Kumar K. Alagiri², Saravanabhavan Thangavel

¹Centre for Stem Cell Research (CSCR), A unit of InStem Bengaluru,Christian Medical College campus, ²Manipal Academy of Higher Education, ³Thiruvalluvar University

Journal (Biochemistry)

Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination

Yesenia Rodriguez, Kevin John Butay*¹, Kedar Sharma*¹, Elizabeth Viverette*¹, Samuel H. Wilson

¹Genome Integrity and Structural Biology Laboratory,National Institute of Environmental Health Sciences, National Institutes of Health

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