All Protocols and Video Articles for scientific research | JoVE

Showing 61 - 72 of 108 videos for "EcoRI"

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Journal (Immunology and Infection)

Rescue and Characterization of Recombinant Virus from a New World Zika Virus Infectious Clone

James Weger-Lucarelli, Nisha K. Duggal, Aaron C. Brault, Brian J. Geiss, Gregory D. Ebel

¹Department of Microbiology, Immunology, and Pathology,Colorado State University, ²Division of Vector-Borne Diseases,Centers for Disease Control and Prevention

Journal (Genetics)

Protocols for C-Brick DNA Standard Assembly Using Cpf1

Shi-Yuan Li, Guo-Ping Zhao, Jin Wang

¹Institute of Plant Physiology and Ecology, Shanghai Insititutes for Biological Sciences,Chinese Academy of Sciences

Journal (Bioengineering)

Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials

Keith C. Heyde*^1,2, Felicia Y. Scott*³, Sung-Ho Paek, Ruihua Zhang, Warren C. Ruder⁴

¹Department of Mechanical Engineering,Carnegie Mellon University, ²Engineering Science and Mechanics Program,Virginia Polytechnic Institute and State University, ³Department of Biological Systems Engineering,Virginia Polytechnic Institute and State University, ⁴Department of Bioengineering,University of Pittsburgh

Journal (Biochemistry)

Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells

Zheng Ma*¹, Victor Fung*¹, Iván D'Orso

¹Department of Microbiology,The University of Texas Southwestern Medical Center

Journal (Genetics)

Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX

Mohamed I. Hassan*¹, Fern R. McSorley*¹, Kinya Hotta, Christopher N. Boddy

¹Department of Chemistry and Biomolecular Sciences,University of Ottawa, ²School of Biosciences,The University of Nottingham Malaysia Campus

Journal (Immunology and Infection)

Unravelling the Function of a Bacterial Effector from a Non-cultivable Plant Pathogen Using a Yeast Two-hybrid Screen

Katrin Janik, Katja Schlink

¹Department of Molecular Biology - Functional Genomics,Laimburg Research Centre

Journal (Cancer Research)

Conditional Knockdown of Gene Expression in Cancer Cell Lines to Study the Recruitment of Monocytes/Macrophages to the Tumor Microenvironment

Marta H. Kubala², Yves A. DeClerck^2,3

¹Division of Hematology, Oncology and Blood and Marrow Transplantation, Department of Pediatrics,University of Southern California, ²The Saban Research Institute of Children's Hospital Los Angeles, ³Department of Biochemistry and Molecular Medicine,University of Southern California

Journal (Biochemistry)

Combining Wet and Dry Lab Techniques to Guide the Crystallization of Large Coiled-coil Containing Proteins

Jenna K. Zalewski, Simone Heber², Joshua H. Mo, Keith O'Conor, Jeffrey D. Hildebrand, Andrew P. VanDemark

¹Department of Biological Sciences,University of Pittsburgh, ²Institute of Structural Biology,German Research Center for Environmental Health

Journal (Genetics)

Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation

Fettah Erdogan, Cristina Lento, Ayat Yaseen, Roksana Nowroozi-Dayeni, Sasha Kheyson, Gerald F. Audette²

¹Department of Chemistry,York University, ²The Centre for Research on Biomolecular Interactions,York University

Journal (Immunology and Infection)

Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle

Hironori Nishitsuji, Hiromi Yamamoto, Ritsuko Shiina, Keisuke Harada, Saneyuki Ujino, Kunitada Shimotohno

¹Research Center for Hepatitis and Immunology,National Center for Global Health and Medicine, ²Central Pharmaceutical Research Institute,Japan Tobacco Inc.

Journal (Immunology and Infection)

Generating De Novo Antigen-specific Human T Cell Receptors by Retroviral Transduction of Centric Hemichain

Tingxi Guo², Toshiki Ochi*², Munehide Nakatsugawa*², Yuki Kagoya, Mark Anczurowski², Chung-Hsi Wang², Muhammed A. Rahman, Kayoko Saso, Marcus O. Butler², Naoto Hirano²

¹Department of Immunology,University of Toronto, ²Princess Margaret Cancer Centre,University Health Network

Journal (Genetics)

Tools to Study the Role of Architectural Protein HMGB1 in the Processing of Helix Distorting, Site-specific DNA Interstrand Crosslinks

Anirban Mukherjee, Karen M. Vasquez

¹Division of Pharmacology and Toxicology, College of Pharmacy,The University of Texas at Austin

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