All Protocols and Video Articles for scientific research | JoVE

Showing 85 - 96 of 108 videos for "EcoRI"

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Journal (Neuroscience)

Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons

Xiaobei Zhao, Yue Zhou, April M. Weissmiller, Matthew L. Pearn⁴, William C. Mobley, Chengbiao Wu

¹Department of Neurosciences,University of California, San Diego, ²School of Biomedical Engineering and Med-X Research Institute,Shanghai Jiao Tong University, ³Department of Anesthesiology,University of California, San Diego, ⁴VA San Diego Healthcare System

Journal (Biology)

Recombinant Protein Expression for Structural Biology in HEK 293F Suspension Cells: A Novel and Accessible Approach

Nicola Portolano, Peter J. Watson, Louise Fairall, Christopher J. Millard, Charles P. Milano, Yun Song, Shaun M. Cowley, John W.R. Schwabe

¹Department of Biochemistry,University of Leicester

Journal (Biology)

Zinc-finger Nuclease Enhanced Gene Targeting in Human Embryonic Stem Cells

Brigham J. Hartley, Stewart A. Fabb, Ben A.L. Finnin, John M. Haynes, Colin W. Pouton

¹Drug Discovery Biology, Monash Institute of Pharmaceutical Sciences,Monash University

Journal (Medicine)

Focus Formation: A Cell-based Assay to Determine the Oncogenic Potential of a Gene

Angel Alvarez, Gustavo A. Barisone, Elva Diaz

¹Department of Pharmacology,University of California, Davis

Journal (Bioengineering)

Characterization of Complex Systems Using the Design of Experiments Approach: Transient Protein Expression in Tobacco as a Case Study

Johannes Felix Buyel, Rainer Fischer²

¹Institute for Molecular Biotechnology,RWTH Aachen University, ²Institute for Molecular Biology and Applied Ecology,Fraunhofer Gesellschaft

Journal (Neuroscience)

In ovo Expression of MicroRNA in Ventral Chick Midbrain

Carola Huber, A. Alwin Prem Anand, Manfred Mauz, Peter Künstle, Wolfgang Hupp, Bernhard Hirt, Andrea Wizenmann

¹Department of Experimental Embryology, Institute of Anatomy,University of Tübingen

Journal (Biology)

Genome-wide Gene Deletions in Streptococcus sanguinis by High Throughput PCR

Xiuchun Ge, Ping Xu

¹The Philips Institute of Oral and Craniofacial Molecular Biology,Virginia Commonwealth University

Journal (Bioengineering)

A Toolkit to Enable Hydrocarbon Conversion in Aqueous Environments

Eva K. Brinkman, Kira Schipper, Nadine Bongaerts, Mathias J. Voges, Alessandro Abate, S. Aljoscha Wahl

¹Department of Biotechnology,Delft University of Technology, ²Delft Center for Systems and Control,Delft University of Technology

Journal (Bioengineering)

Engineering Adherent Bacteria by Creating a Single Synthetic Curli Operon

Benoît Drogue, Philippe Thomas, Laurent Balvay, Claire Prigent-Combaret, Corinne Dorel

¹UMR CNRS 5557 Ecologie Microbienne, Université Lyon 1,Université de Lyon, ²Département Biosciences, INSA de Lyon,Université de Lyon, ³INSERM U758, Ecole Normale Supérieure de Lyon,Université de Lyon, ⁴Laboratoire de Génie Civil et Ingénierie Environnementale, INSA de Lyon,Université de Lyon

Journal (Medicine)

DNA Vector-based RNA Interference to Study Gene Function in Cancer

Daniel B. Stovall, Meimei Wan, Qiang Zhang, Purnima Dubey, Guangchao Sui

¹Department of Cancer Biology and Comprehensive Cancer Center,Wake Forest University School of Medicine, ²Department of Pathology and Comprehensive Cancer Center,Wake Forest University School of Medicine

Journal (Biology)

The Green Monster Process for the Generation of Yeast Strains Carrying Multiple Gene Deletions

Yo Suzuki, Jason Stam, Mark Novotny, Nozomu Yachie, Roger S. Lasken, Frederick P. Roth⁴

¹Department of Synthetic Biology and Bioenergy,J. Craig Venter Institute, ²Department of Microbial and Environmental Genomics,J. Craig Venter Institute, ³Donnelly Centre & Department of Molecular Genetics,University of Toronto, ⁴Lunenfeld Research Institute,Mt Sinai Hospital

Journal (Biology)

Isolation of Ribosome Bound Nascent Polypeptides in vitro to Identify Translational Pause Sites Along mRNA

Sujata S. Jha, Anton A. Komar

¹Center for Gene Regulation in Health and Disease, Department of Biological, Geological and Environmental Sciences,Cleveland State University

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