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Krebsforschung
Lentiviral Packaging and Viral Titering for Genome-Scale shRNA Screens (Video) | JoVE
Published: August 25, 2023
doi:
10.3791/200477
Brittany Turner-Ivey
,
Jody Fromm Longo
,
Dorea P. Jenkins
,
Stephen T. Guest
,
Steven L. Carroll
2
1
Hollings Cancer Center
,
Medical University of South Carolina
,
2
Department of Pathology and Laboratory Medicine
,
Medical University of South Carolina
,
3
Department of Computational Medicine and Bioinformatics
,
University of Michigan, Ann Arbor
Tags
Lentiviral Packaging
Viral Titering
Genome-scale ShRNA Screens
293T Cells
Packaging Plasmid Mix
Plasmid Barcode Library
Transfection Reagent
Viral Particles
Viral Titering
Cationic Polymer
Tumor Cell Growth Media
Tumor Cells
Lentivirus
check_url/de/200477?article_type=t
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Lentiviral Packaging and Viral Titering for Genome-Scale shRNA Screens.
J. Vis. Exp.
(Pending Publication), e200477, doi:10.3791/200477 (2023).
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