Assembling Ha-CoV-2 Particles by Cotransfection of HEK293T Cells for Quantification of SARS-CoV-2 Variants

doi:10.3791/201201

JoVE Journal > Immunology and Infection

Immunologie und Infektion

Assembling Ha-CoV-2 Particles by Cotransfection of HEK293T Cells for Quantification of SARS-CoV-2 Variants

Published: September 08, 2023

doi:

10.3791/201201

Linda Chilin, Brian Hetrick, Yuntao Wu

¹Center for Infectious Disease Research, School of Systems Biology,George Mason University

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Keywords: HEK293T Cells DMEM FBS Penicillin Streptomycin Trypan Blue Cell Counting Cell Culture Cotransfection Viral Particle Assembly SARS-CoV-2 Variants Ha-CoV-2 Pseudovirus Centrifugation Filtration Storage

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Assembling Ha-CoV-2 Particles by Cotransfection of HEK293T Cells for Quantification of SARS-CoV-2 Variants. J. Vis. Exp. (Pending Publication), e201201, doi:10.3791/201201 (2023).

Assembling Ha-CoV-2 Particles by Cotransfection of HEK293T Cells for Quantification of SARS-CoV-2 Variants

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