Immunofluorescence Fixation, Staining, and Clearing of Encapsulated Spheroids for Confocal Microscopy

JoVE Journal > Bioengineering

Biotechnik

Immunofluorescence Fixation, Staining, and Clearing of Encapsulated Spheroids for Confocal Microscopy

Published: September 22, 2023

doi:

10.3791/201261

Joseph Bruns, Shabnam Nejat, Allison Faber, Silviya P. Zustiak

¹Biomedical Engineering Department,Saint Louis University

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Keywords: Immunofluorescence Fixation Staining Clearing Encapsulated Spheroids Confocal Microscopy Hydrogel PBS Fixative Primary Antibodies Nestin SOX2 Formamide Z-stack Maximum Projection DAPI Stem Cell Marker

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Immunofluorescence Fixation, Staining, and Clearing of Encapsulated Spheroids for Confocal Microscopy. J. Vis. Exp. (Pending Publication), e201261, doi:10.3791/201261 (2023).

Immunofluorescence Fixation, Staining, and Clearing of Encapsulated Spheroids for Confocal Microscopy

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