In this video, we present a photoacoustic flow cytometric detection of ovarian cancer cells bearing nanoparticles in a circulating in vitro flow chamber. This non-invasive technique successfully identifies circulating tumor cells in a flow system utilizing a contrast agent.
Protocol
Flow System Architecture 1. Flow chamber construction Using the provided Solid Works file, 3D print the flow tank using ABS thermoplastic or PLA plastic. Dimensions are provided below if SolidWorks is unavailable. Figure 1A shows a representation of this model. The body of the flow tank is 2.5 cm x 1.5 cm x 7.5 cm. The far ends of the flow tank include holes approximately 5 mm in diameter to allow for the entry of tubing co…
Representative Results
Figure 1. Representative images of photoacoustic flow cytometry system and flow chamber. (A) Detailed view of the 3D printed flow chamber. (B) Diagram of PAFC system. (C) Flow system architecture: SP = syringe pump; DAQ/FPGA = data acquisition/field programmable gate array; Ob = objective len…
Offenlegungen
The authors have nothing to disclose.
Materials
3D Printed Tank
Custom-made
Acquisition Card
National Instruments
PXIe-5170R
250 MS/s, 8-Channel, 14-bit
Alconox
Sigma-Aldrich
242985-1.8KG
Detergent used for cleaning glassware.
Amicon Ultra-15 Centrifugal Filters
Millipore
UFC903024
Amicon Ultra-4 Centrifugal Filters
Millipore
UFC803024
Data Acquisition software
National Instruments
NI LabVIEW 2017 (32-bit)
LabVIEW used to synchronize laser pulses with data acquisition.
Data Processing Software
Mathworks
Matlab R2016a
Reconstructions and graphs produced using Matlab software.
In Vitro Photoacoustic Flow Cytometry: A Non-invasive Flow Photoacoustic Technique to Detect Nanoparticle-bearing Circulating Ovarian Cancer Cells. J. Vis. Exp. (Pending Publication), e20369, doi: (2023).