Intracerebroventricular Injection in Mouse: An Ommaya Mediated Direct Drug Administration Method to Deliver Drugs to Cerebral Ventricles of Mouse Brain
This video demonstrates ommaya mediated intracerebroventricular injection in a mouse model. The method enables localized delivery of potential drug candidates and evades the blood-brain barrier.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Murine Ommaya Assembly and Implantation Preparation of station Disinfect the station surface. Place a blue covering over the surface and keep ready all sterilized tools and supplies indicated in Figure 1. Presurgical procedure Apply analgesia (Bup-SR)…
Representative Results
Figure 1: An example of a workstation setup for performing the Murine Ommaya implantation in mice. (1) Gas anesthesia machine/vaporizer. (2) Sterile blue paper drape covering a stereotaxic stand. (3) Stereotaxic device (stand/stage, ear bars, nose cone). (4) Microdrill. (5) Magnifying glass with light. (6) Sterile cotton-tipped applicator sticks with sterile saline rinse container. (7) Hydrogen peroxide. (8) Bead sterilizer. <img alt="Fi…
Offenlegungen
The authors have nothing to disclose.
Materials
1 mm spacer disc
Alzet, Durect Corporation
#0008670
Spacer disc only
4-0 ethilon nylon suture
Any vendor
Automatic syringe pumps
Harvard Syringe Pumps (or any vendor)
#70-4505
Pump 11 Elite
Bead sterilizer
Braintree Scientific Inc. (or any vendor)
#GER 5287-120V
Germinator 500
Buprenorphine Sustained-Release (Bup-SR)
Zoopharm
DEA controlled
Cyanoacrylate sterile adhesive
Any vendor
Gas inhalation anestehsia system
VeteEquip
#901812
COMPAC5
Hamilton microliter syringes
Hamilton
10, 25, 50, and 100 μL;
30G for cisterna magna injection
Intracerebroventricular Injection in Mouse: An Ommaya Mediated Direct Drug Administration Method to Deliver Drugs to Cerebral Ventricles of Mouse Brain. J. Vis. Exp. (Pending Publication), e20612, doi: (2023).