Langendorff Retrograde Heart Perfusion: An Ex Vivo Technique to Perfuse Isolated Mouse Heart

Published: April 30, 2023

Abstract

Source: Wu, K. et al. Modifications of the Langendorff Method for Simultaneous Isolation of Atrial and Ventricular Myocytes from Adult Mice. J. Vis. Exp. (2021)

In this video, we perform Langendorff retrograde perfusion of an isolated mouse heart. During the procedure, the cardiac activity of the heart is maintained by perfusing it retrogradely with a perfusion solution via the coronary arteries using a cannula inserted into the ascending aorta. The model is used to study the cardiac pathophysiology of the heart.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Solution and Perfusion Apparatus Preparation Assemble a constant flow Langendorff apparatus (commercially available or self-made). Figure 1 provides a simple schematic. Prepare the solutions according to the reagents given in Table 1 and Table …

Representative Results

Figure 1. Schematic diagram of an assembled modified Langendorff system. This system is economical and portable for the users. It has two parts of plastic tubing-one for the water (inner diameter, 8 mm) and one for the perfusate (inner diameter, 1.5 mm)-of which the distal end is connected to a PE medical three-way stopcock with a Luer lock. A glass bottle containing water an…

Offenlegungen

The authors have nothing to disclose.

Materials

2,3-butanedione monoxime (BDM)  Sigma-Aldrich  31550
CaCl2  Sigma-Aldrich  C4901
Glucose  Sigma-Aldrich  G7528
HEPES  Sigma-Aldrich  H3375
KCl  Sigma-Aldrich  P9333
KH2PO Sigma-Aldrich  P5655
KHCO3  Sigma-Aldrich  237205
MgCl2  Sigma-Aldrich  M8266
MgSO4  Sigma-Aldrich  M7506
Na2HPO4 Sigma-Aldrich  S7907
NaCl  Sigma-Aldrich  S7653
NaHCO3  Sigma-Aldrich  S5761
Peristaltic pump  Longerpump  BT100-2J
Taurine  Sigma-Aldrich  T0625
Water bath  JULABO  ED (v.2)

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Langendorff Retrograde Heart Perfusion: An Ex Vivo Technique to Perfuse Isolated Mouse Heart. J. Vis. Exp. (Pending Publication), e20749, doi: (2023).

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