Acute Biliary Pancreatitis Model: A Method to Generate Acute Biliary Pancreatitis Mouse Model via Pancreatic Duct Infusion

Abstract

Source: Serra, M. B. et al. Sodium Taurocholate Induced Severe Acute Pancreatitis in C57BL/6 Mice. J. Vis. Exp. (2021)

This video describes the procedure for the generation of a mouse model with acute biliary pancreatitis via pancreatic infusion of sodium taurocholate. This model helps study pathophysiological changes induced by sodium taurocholate in the pancreas.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Laparotomy

1. Anesthetize animals with xylazine (10 mg/kg) and ketamine solution (80 mg/kg), subcutaneously (0.1 mL/10g of body weight) using a 1 mL syringe and 13 x 0.45 mm needle 26 G ½. Check for sufficient anesthesia depth by pinching the toe. Control body temperature using heated pads. Ensure that all surgical materials are sterile.
2. Clean the abdominal area with 5% povidone-iodine solution and use a trimmer to remove hair between the chest and lower ....

Results

Figure 1: Schematics of severe acute pancreatitis induction by 2.5% sodium taurocholate in C57BL/6 mice. (A) gallbladder; (B) common bile duct; (C) pancreatic duct; .......

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
0.4 mm needle	INTRAG MEDICAL TECH	90183210	30G
0.54 mm polyethylene tube	Tygon	730010
Styrofoam block
Masking tape for mounting the mouse	Missner	1236
Infusion pump scheduled to 10µL/min.	Havard aparatus-Peristaltic Pump Series	MA1 55-7766	 Model 66 Small Peristaltic
Scissors and forceps
Antiseptic providine iodine	Pfizer	12086OR	Antisepsis
70% ethanol	SIGMA	459836	Mix 700 mL 100% ethanol with 300 mL dH2O
Razor blade	Lord	bdk9a1ghk6	For trichotomy
Sodium taurocholate	Sigma-Aldrich	86339- 1G	CAS NUMBER- 345909-26-4
Microvessel clip	Medicon Surgical	56.87.35	Approximator, opening 4.0 mm, closing pressure 30-40 g
6-0 prolene	Bioline	5162	Suture line
Ketamin NP (cloridrato de dextrocetamina) 50mg/mL	Cristália
Xilazine 2%	Syntec
Sterile saline solution (0.9% (wt/vol) saline)	Farmace	105851
Methyl Blue	Sigma-Aldrich Chemicals	M5528
Desmarres retractor 13 mm width	ROBOZ	RS-6672