A Mouse Tail Snip Procedure for Peripheral Blood Collection and Plasma Isolation

Published: November 30, 2023

Abstract

Source: Coats, J. S. et al., Expression of Exogenous Cytokine in Patient-derived Xenografts via Injection with a Cytokine-transduced Stromal Cell Line. J. Vis. Exp. (2017)

In this video, we demonstrate a tail-snipping procedure in a mouse model to collect peripheral blood. The collected blood is subsequently subjected to centrifugation to separate plasma, which can be used for further experiments.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Serial Blood Collection and Plasma Monitoring for Exogenous Human Cytokine in Mice Blood collection via tail snip Disinfect the biosafety hood and assemble the mouse restrainer, scissors, and other tools/supplies for the procedure. Place the mouse in a re…

Offenlegungen

The authors have nothing to disclose.

Materials

Animal Implantable Nano Transponder with Canula Trovan ID-100B(1.25)
EMLA lidocaine anesthetic cream (obtain by presciption through animal care facility) perscription perscription
BD ½ cc LO-DOSE U-100 Insulin Syringe 28G½ BD #329461
BD Microtainer Tubes with K2EDTA BD #365974
Centrifuge Beckman Coulter Alegra X-15R
FisherBrand Capillary Tubes (Heparinized) Fisher Scientific 22-260-950
Hemocytometer Fisher 02-671-6
MACSQuant Analyzer 10 Miltenyi Biotec 130-096-343
Mouse Pie Cage Braintree Scientific, Inc. MPC-1
Mouse Tail Illuminator Restrainer Braintree Scientific, Inc. MTI STD
Pistol Grip Implanter Trovan IM-300(1.25)
"NSG mice" NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ, male or female, age 6 – 8 weeks JAX Mice # 005557

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Diesen Artikel zitieren
A Mouse Tail Snip Procedure for Peripheral Blood Collection and Plasma Isolation. J. Vis. Exp. (Pending Publication), e21836, doi: (2023).

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