This video demonstrates a flow cytometry-based phagocytosis assay involving labeled and opsonized Plasmodium falciparum-infected erythrocytes by monocytes. Flow cytometry identifies fluorescence in monocytes, confirming the successful identification of opsonized erythrocytes through phagocytosis.
Protocol
All procedures involving sample collection have been performed in accordance with the institute's IRB guidelines. 1. Parasite culture NOTE: Follow the local regulations for human pathogens handling. Maintain Plasmodium falciparum parasites according to the standard protocol as described before in the parasite culture medium (see Table of Materials). Keep the par…
Representative Results
Figure 1: Fc receptors expressed on the THP-1 cell surface. (A) Fcγ-receptor III/CD16 (red). (B) Fcγ-receptor II/CD32 (green). C. Fcγ-receptor I/CD64 (orange). Unlabeled cells are shown in blue. Figure 2: Phagocytosis assay flow…
Offenlegungen
The authors have nothing to disclose.
Materials
96 well cell culture plates, round bottom with lid
Corning
3799
Any similar plate can be used, make sure it is compatible with the flow cytometer instrument you intend to use