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JoVE Journal Biology
Proteomics to Identify Proteins Interacting with P2X2 Ligand-Gated Cation Channels
 

Proteomics to Identify Proteins Interacting with P2X2 Ligand-Gated Cation Channels

Article DOI: 10.3791/1178-v • 16:37 min • May 18th, 2009
May 18th, 2009 •
1, 2, 3, 1
1Department of Physiology, David Geffen School of Medicine, University of California, Los Angeles, 2Department of Anesthesiology, David Geffen School of Medicine, University of California, Los Angeles, 3Department of Anesthesiology, Medicine and Physiology, David Geffen School of Medicine, University of California, Los Angeles

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Summary
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We describe a simple protocol to identify brain proteins that bind to the full length C terminus of ATP-gated P2X2 receptors. The extension and systematic application of this approach to all P2X receptors is expected to lead to a better understanding of P2X receptor signaling.

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Proteomics Proteins P2X2 Ligand-gated Cation Channels Ligand-gated Ion Channels Synaptic Communication Nervous System Cys Loop Glutamate-gated Channels P2X Receptor Channels Transmitter Binding Pore Opening Ions Flow Electrochemical Gradients Calcium Ions Downstream Signaling Roles Gene Regulation Protein Signaling Complexes C-terminal Aspects P2X2 Receptor Cytosolic Domain ATP-gated Cation Channels Subunits Excitatory Synaptic Transmission Neurotransmitter Release Excitable Cells Non-excitable Cells Neuronal Signaling Inflammation Cardiovascular Function
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